Referenced in: none

Automatically associated channels: KChIP2 , Kv1.4 , Kv3.1 , Kv4.2

Title: Ito channels are octomeric complexes with four subunits of each Kv4.2 and K+ channel-interacting protein 2.

Authors: Leo A Kim, Johannes Fürst, Margaret H Butler, Shuhua Xu, Nikolaus Grigorieff, Steve A N Goldstein

Journal, date & volume: J. Biol. Chem., 2004 Feb 13 , 279, 5549-54

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/14623880

Abstract
Mammalian voltage-gated K+ channels are assemblies of pore-forming alpha-subunits and modulating beta-subunits. To operate correctly, Kv4 alpha-subunits in the heart and central nervous system require recently identified beta-subunits of the neuronal calcium sensing protein family called K+ channel-interacting proteins (KChIPs). Here, Kv4.2.KChIP2 channels are purified, integrity of isolated complexes confirmed, molar ratio of the subunits determined, and subunit valence established. A complex has 4 subunits of each type, a stoichiometry expected for other channels employing neuronal calcium sensing beta-subunits.