Referenced in: none

Automatically associated channels: Kv2.1

Title: Solution structure and functional characterization of SGTx1, a modifier of Kv2.1 channel gating.

Authors: Chul Won Lee, Sunghwan Kim, Soung Hun Roh, Hiroshi Endoh, Yoshio Kodera, Tadakazu Maeda, Toshiyuki Kohno, Julia M Wang, Kenton J Swartz, Jae Il Kim

Journal, date & volume: Biochemistry, 2004 Feb 3 , 43, 890-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/14744131

Abstract
SGTx1 is a peptide toxin isolated from the venom of the spider Scodra griseipes that has been shown to inhibit outward K(+) currents in rat cerebellar granule neurons. Although its amino acid sequence is known to be highly (76%) homologous with that of hanatoxin (HaTx), a well-characterized modifier of Kv2.1 channel gating, the structural and functional characteristics of SGTx1 remain largely unknown. Here we describe the NMR solution structure of SGTx1, the mechanism of its interaction with Kv2.1 channels, and its effect on channel activity once bound. The NMR structure of SGTx1 contains a molecular fold closely resembling the "inhibitor cystine knot" of HaTx, which is composed of an antiparallel beta-sheet and four chain reversals stabilized by three disulfide bonds. Functionally, SGTx1 reversibly inhibited K(+) currents in oocytes expressing Kv2.1 channels. Moreover, generation of steady-state activation curves showed that, consistent with other gating modifiers, SGTx1 acted by shifting the activation of the channel to more depolarized voltages. Thus, the surface profile and mechanism of action of SGTx1 are similar to those of HaTx. Still, detailed comparison of SGTx1 with HaTx revealed differences in binding affinity and conformational homogeneity that result from differences in the charge distribution at the binding surface and in the amino acid composition of the respective beta-hairpin structures in the peptides.