Referenced in: none

Automatically associated channels: Kv7.1

Title: Filamin C: a novel component of the KCNE2 interactome during hypoxia.

Authors: Annika Neethling, Jomien Mouton, Ben Loos, Valerie Corfield, Carin de Villiers, Craig Kinnear

Journal, date & volume: Cardiovasc J Afr, 2015 Jan-Feb , 27, 4-11

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/26956495

Abstract
KCNE2 encodes for the potassium voltage-gated channel, KCNE2. Mutations in KCNE2 have been associated with long-QT syndrome (LQTS). While KCNE2 has been extensively studied, the functions of its C-terminal domain remain inadequately described. Here, we aimed to elucidate the functions of this domain by identifying its protein interactors using yeast two-hybrid analysis.The C-terminal domain of KCNE2 was used as bait to screen a human cardiac cDNA library for putative interacting proteins. Co-localisation and co-immunoprecipitation analyses were used for verification.Filamin C (FLNC) was identified as a putative interactor with KCNE2. FLNC and KCNE2 co-localised within the cell, however, a physical interaction was only observed under hypoxic conditions.The identification of FLNC as a novel KCNE2 ligand not only enhances current understanding of ion channel function and regulation, but also provides valuable information about possible pathways likely to be involved in LQTS pathogenesis.