Referenced in: none

Automatically associated channels: Kir2.3 , SK3

Title: Altered distribution of small-conductance calcium-activated potassium channel SK3 in Hirschsprung's disease.

Authors: David Coyle, Anne Marie O'Donnell, Prem Puri

Journal, date & volume: J. Pediatr. Surg., 2015 Oct , 50, 1659-64

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/25783396

Abstract
SK3 channels are voltage-independent Ca(2+)-dependent K(+) channels that play a key role in regulating smooth muscle membrane potential during purinergic inhibitory neurotransmission in the colon. Dysmotility problems are common after a properly performed pull-through operation for Hirschsprung's disease (HSCR). We hypothesised that ganglionic bowel just proximal to the transition zone is abnormal and designed this study to investigate SK3 channel expression in HSCR.Entire resected bowel specimens were collected at the time of pull-through surgery for HSCR (n=6). Control colonic specimens were obtained at the time of colostomy closure in patients following anorectoplasty (n=6). SK3 protein expression was assessed qualitatively using immunofluorescence with confocal microscopy and quantitatively using western blot (WB) analysis.Positive SK3 immunofluorescence was seen in the mucosa and in all three smooth muscle layers and the myenteric plexus in control specimens. SK3 immunofluorescence co-localised with PDGFRα. A band was detected at ~70kDa on WB. SK3 protein expression was barely detectable in aganglionic tissue and markedly reduced in the ganglionic bowel of 3 patients with HSCR compared to controls.Decreased SK3 expression in ganglionic bowel may explain the basis of persistent bowel symptoms in some patients following a properly performed pull-through operation for HSCR.