Referenced in: none

Automatically associated channels: Kv10.1

Title: Total synthesis of mambalgin-1/2/3 by two-segment hydrazide-based native chemical ligation.

Authors: Huan Lan, Kun Wu, Yong Zheng, Man Pan, Yi-Chao Huang, Shuai Gao, Qing-Yun Zheng, Ji-Shen Zheng, Yi-Ming Li, Bailong Xiao, Lei Liu

Journal, date & volume: J. Pept. Sci., 2016 May , 22, 320-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/26991634

Abstract
Mambalgins are a class of 57-residue polypeptide toxins isolated from the venom of the African mamba. They exhibit potent analgesic effects by inhibiting the acid-sensing ion channels. Classified as members of the family of three-finger toxins, mambalgins contain four pairs of disulfide bridges that help to stabilize the three-finger scaffold. Here, we report the chemical synthesis of functional mambalgin-1/2/3 by using one-step two-segment hydrazide-based native chemical ligation. The two-segment ligation approach reported here may enable efficient production of mambalgin toxins. These synthetic mambalgins are useful compounds for development of diagnostic or therapeutic reagents. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.