Referenced in: none

Automatically associated channels: Kv10.1

Title: A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum.

Authors: Lichang Wu, Yu Sun, Liqiao Ma, Jun Zhu, Baoxia Zhang, Qingjie Pan, Yuyin Li, Huanqi Liu, Aipo Diao, Yinchuan Li

Journal, date & volume: Sci Rep, 2016 , 6, 27332

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/27265833

Abstract
The Bestrophin family has been characterized as Cl(-) channels in mammals and Na(+) channels in bacteria, but their exact physiological roles remian unknown. In this study, a natural C-terminally truncated variant of mouse Bestrophin 3 (Best3V2) expression in myoblasts and muscles is demonstrated. Unlike full-length Best3, Best3V2 targets the two important intracellular Ca stores: the lysosome and the ER. Heterologous overexpression leads to lysosome swelling and renders it less acidic. Best3V2 overexpression also results in compromised Ca(2+) release from the ER. Knocking down endogenous Best3 expression in myoblasts makes these cells more excitable in response to Ca(2+) mobilizing reagents, such as caffeine. We propose that Best3V2 in myoblasts may work as a tuner to control Ca(2+) release from intracellular Ca(2+) stores.