Referenced in: none

Automatically associated channels: SK3

Title: The pro-proliferative effects of nicotine and its underlying mechanism on rat airway smooth muscle cells.

Authors: Fang He, Bing Li, Zhuxiang Zhao, Yumin Zhou, Guoping Hu, Weifeng Zou, Wei Hong, Yimin Zou, Changbin Jiang, Dongxing Zhao, Pixin Ran

Journal, date & volume: PLoS ONE, 2014 , 9, e93508

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24690900

Abstract
Recent studies have shown that nicotine, a major component of cigarette smoke, can stimulate the proliferation of non-neuronal cells. Cigarette smoking can promote a variety of pulmonary and cardiovascular diseases, such as chronic obstructive pulmonary disease (COPD), atherosclerosis, and cancer. A predominant feature of COPD is airway remodeling, which includes increased airway smooth muscle (ASM) mass. The mechanisms underlying ASM remodeling in COPD have not yet been fully elucidated. Here, we show that nicotine induces a profound and time-dependent increase in DNA synthesis in rat airway smooth muscle cells (RASMCs) in vitro. Nicotine also significantly increased the number of RASMCs, which was associated with the increased expression of Cyclin D1, phosphorylation of the retinoblastoma protein (RB) and was dependent on the activation of Akt. The activation of Akt by nicotine occurred within minutes and depended upon the nicotinic acetylcholine receptors (nAchRs). Activated Akt increased the phosphorylation of downstream substrates such as GSK3β. Our data suggest that the binding of nicotine to the nAchRs on RASMCs can regulate cellular proliferation by activating the Akt pathway.