PubMed 25701762

Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv10.1

Title: Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores.

Authors: Strahinja Križak, Ljiljana Nikolić, Marina Stanić, Milan Žižić, Joanna Zakrzewska, Miroslav Zivić, Natasa Todorović

Journal, date & volume: Res. Microbiol., 2015 Apr , 166, 162-73

PubMed link:

We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.