Referenced in: none

Automatically associated channels: TRP , TRPV , TRPV1

Title: The molecular pathway of ATP-sensitive potassium channel in endothelial cells for mediating arteriole relaxation.

Authors: Xuejun Chen, Wenzhi Han, YanFang Zhang, Wenyu Cui, ZhiYuan Pan, Xin Jin, Chaoliang Long, Hai Wang

Journal, date & volume: Life Sci., 2015 Sep 15 , 137, 164-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/26188291

Abstract
The endothelial molecular pathway of a new ATP-sensitive potassium channel (KATP) opener natakalim was investigated in mesenteric arterioles of rats.A DMT wire myograph was used to evaluate the vasorelaxation effects of natakalim. Ca(2+) responses of endothelial cells induced by natakalim were measured by laser confocal fluorescence microscopy. NO assay kits and Western blotting were used.The new KATP opener natakalim significantly produced endothelium-dependent arteriolar dilation and increased endothelial cell intracellular calcium concentration ([Ca(2+)]i) as well as NO release, which could be inhibited by SB366791 and capsazepine, the specific TRPV1 blockers. Additionally, down-regulation of endothelial TRPV1 by RNA interference inhibited the Ca(2+) influx induced by natakalim.These results suggest that endothelial KATP mediated natakalim-induced vasorelaxation through increasing [Ca(2+)]i and NO production. Activation of endothelial TRPV1 channels and subsequent Ca(2+) entry, and NO release at least partly contribute to endothelium-dependent vasorelaxation induced by natakalim.