PubMed 24941800

Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kir2.3

Title: [Diammonium glycyrrhizinate promotes aquaporin-5 in LPS-induced acute lung injury via inactivation of NF-kappaB].

Authors: Min Xiao, Tao Zhu, Tao Wang, Fu-qiang Wen

Journal, date & volume: Sichuan Da Xue Xue Bao Yi Xue Ban, 2014 May , 45, 376-9, 404

PubMed link:

To determine the therapeutic value and associated mechanism of diammonium glycyrrhizinate (DG) on the expression of AQP-5 in lipapolysacchairides (LPS)-induced acute lung injury in mice.Thirty male BALB/c mice were randomly divided into three groups equally: Control, LPS+DG and LPS. HE staining and lung injury score system were used to evaluate the pathological changes in the lung tissues. Wet to dry ratio (W/D) was used to measure the degree of lung edema. RT-PCR and Western blot were obtained to measure AQP-5 expression. Total NF-kappaB p65 and phospho-NF-kappaB p65 (p-NF-kappaB p65) were evaluated by Western blot.After 3 days of LPS intratracheal injection, severe pathological changes, increased W/D, down-regulated AQP-5 expression and increased p-NF-kappaB p65/total NF-kappaB p65 were observed. Compared with mice in the LPS group, mice in the LPS+DG group had more significantly ameliorated pathological changes and increased W/ D, up-regulated AQP-5 expression, and reduced p-NF-kappaB p65/total NF-kappaB p65.DG up-regulates AQP-5 in vivo, possibly resulting from inactivation of NF-kappaB.