Referenced in: none

Automatically associated channels: Kv10.1

Title: Immunohistochemical and calcium imaging methods in wholemount rat retina.

Authors: Allison Sargoy, Steven Barnes, Nicholas C Brecha, Luis Pérez De Sevilla Müller

Journal, date & volume: J Vis Exp, 2014 , , e51396

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/25349920

Abstract
In this paper we describe the tools, reagents, and the practical steps that are needed for: 1) successful preparation of wholemount retinas for immunohistochemistry and, 2) calcium imaging for the study of voltage gated calcium channel (VGCC) mediated calcium signaling in retinal ganglion cells. The calcium imaging method we describe circumvents issues concerning non-specific loading of displaced amacrine cells in the ganglion cell layer.