Referenced in: none

Automatically associated channels: Nav1.9 , Slo1

Title: Expression and functional role of Nav1.9 sodium channel in cartwheel cells of the dorsal cochlear nucleus.

Authors: Zhiyu Yan, Yanjun Xu, Min Liang, Xiaowei Ren

Journal, date & volume: Mol Med Rep, 2015 Mar , 11, 1833-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/25385328

Abstract
In the central auditory system, cartwheel cells (CWCs) are a group of interneurons in the dorsal cochlear nucleus (DCN). While other DCN neurons respond to stimuli with a simple discharge pattern of single action potentials (SAPs), CWCs respond with complex action potentials (CAPs), consisting of SAPs superimposed on a slow depolarization. The CAPs in CWCs may participate in various auditory or non‑auditory signaling processing but its intrinsic mechanisms are largely unknown. In the present study, in vitro whole‑cell current clamp recordings on neonatal mice brain slices were used to demonstrate that CWCs respond to brief voltage stimulation with CAPs. Western blotting and immunohistochemistry were also utilized to demonstrate that Nav1.9 was expressed in the CWCs. Finally, when Nav1.9 was genetically silenced, CWCs responded to voltage stimulation with SAPs, not CAPs. The results strongly suggested that Nav1.9 was expressed and functionally contributed to the signaling processing in the central auditory pathway.