Referenced in: none

Automatically associated channels: Kv7.1 , Slo1

Title: Marine n-3 PUFAs modulate IKs gating, channel expression, and location in membrane microdomains.

Authors: Cristina Moreno, Alicia de la Cruz, Anna Oliveras, Sanjay R Kharche, Miriam Guizy, Núria Comes, Tomas Stary, Carlotta Ronchi, Marcella Rocchetti, Isabelle Baró, Gildas Loussouarn, Antonio Zaza, Stefano Severi, Antonio Felipe, Carmen Valenzuela

Journal, date & volume: Cardiovasc. Res., 2015 Feb 1 , 105, 223-32

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/25497550

Abstract
Polyunsaturated fatty n-3 acids (PUFAs) have been reported to exhibit antiarrhythmic properties. However, the mechanisms of action remain unclear. We studied the electrophysiological effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on IKs, and on the expression and location of Kv7.1 and KCNE1.Experiments were performed using patch-clamp, western blot, and sucrose gradient techniques in COS7 cells transfected with Kv7.1/KCNE1 channels. Acute perfusion with both PUFAs increased Kv7.1/KCNE1 current, this effect being greater for DHA than for EPA. Similar results were found in guinea pig cardiomyocytes. Acute perfusion of either PUFA slowed the activation kinetics and EPA shifted the activation curve to the left. Conversely, chronic EPA did not modify Kv7.1/KCNE1 current magnitude and shifted the activation curve to the right. Chronic PUFAs decreased the expression of Kv7.1, but not of KCNE1, and induced spatial redistribution of Kv7.1 over the cell membrane. Cholesterol depletion with methyl-β-cyclodextrin increased Kv7.1/KCNE1 current magnitude. Under these conditions, acute EPA produced similar effects than those induced in non-cholesterol-depleted cells. A ventricular action potential computational model suggested antiarrhythmic efficacy of acute PUFA application under IKr block.We provide evidence that acute application of PUFAs increases Kv7.1/KCNE1 through a probably direct effect, and shows antiarrhythmic efficacy under IKr block. Conversely, chronic EPA application modifies the channel activity through a change in the Kv7.1/KCNE1 voltage-dependence, correlated with a redistribution of Kv7.1 over the cell membrane. This loss of function may be pro-arrhythmic. This shed light on the controversial effects of PUFAs regarding arrhythmias.