PubMed 25615535

Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Nav1 , Nav1.2 , SK3

Title: The Nav1.2 channel is regulated by GSK3.

Authors: Thomas F James, Miroslav N Nenov, Norelle C Wildburger, Cheryl F Lichti, Jonathan Luisi, Fernanda Vergara, Neli I Panova-Electronova, Carol L Nilsson, Jai S Rudra, Thomas A Green, Demetrio Labate, Fernanda Laezza

Journal, date & volume: Biochim. Biophys. Acta, 2015 Apr , 1850, 832-44

PubMed link:

Phosphorylation plays an essential role in regulating voltage-gated sodium (Na(v)) channels and excitability. Yet, a surprisingly limited number of kinases have been identified as regulators of Na(v) channels. We posited that glycogen synthase kinase 3 (GSK3), a critical kinase found associated with numerous brain disorders, might directly regulate neuronal Na(v) channels.We used patch-clamp electrophysiology to record sodium currents from Na(v)1.2 channels stably expressed in HEK-293 cells. mRNA and protein levels were quantified with RT-PCR, Western blot, or confocal microscopy, and in vitro phosphorylation and mass spectrometry to identify phosphorylated residues.We found that exposure of cells to GSK3 inhibitor XIII significantly potentiates the peak current density of Na(v)1.2, a phenotype reproduced by silencing GSK3 with siRNA. Contrarily, overexpression of GSK3β suppressed Na(v)1.2-encoded currents. Neither mRNA nor total protein expression was changed upon GSK3 inhibition. Cell surface labeling of CD4-chimeric constructs expressing intracellular domains of the Na(v)1.2 channel indicates that cell surface expression of CD4-Na(v)1.2 C-tail was up-regulated upon pharmacological inhibition of GSK3, resulting in an increase of surface puncta at the plasma membrane. Finally, using in vitro phosphorylation in combination with high resolution mass spectrometry, we further demonstrate that GSK3β phosphorylates T(1966) at the C-terminal tail of Na(v)1.2.These findings provide evidence for a new mechanism by which GSK3 modulates Na(v) channel function via its C-terminal tail.These findings provide fundamental knowledge in understanding signaling dysfunction common in several neuropsychiatric disorders.