Referenced in: none

Automatically associated channels: TRP , TRPA

Title: Off-response in ASH neurons evoked by CuSO4 requires the TRP channel OSM-9 in Caenorhabditis elegans.

Authors: Wei Wang, Zi-Jing Xu, Ya-Qian Wu, Li-Wei Qin, Zhao-Yu Li, Zheng-xing Wu

Journal, date & volume: Biochem. Biophys. Res. Commun., 2015 Jun 5 , 461, 463-8

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/25871795

Abstract
The off-response of ASH neurons had been overlooked until the microfluidic devices were introduced for in vivo imaging of neuronal activity in Caenorhabditis elegans. The mechanisms of ASH off-response were completely unknown. Here we monitored ASH off-response to CuSO4 stimulation by use of microfluidic device and genetically encoded calcium indicator (GECI) - Case12. We found ASH neurons exhibited a multiphasic response to 10 mM and 50 mM CuSO4 of 30-s stimulation duration. ASH off-responding to Cu(2+) had been dramatically reduced in goa-1, mod-5, trpa-1 and egl-8 mutants. Moreover, in osm-9 mutants ASH off-response was completely eliminated. Neuron-specific rescue of osm-9 in ASH neurons restored the off-response and the normal avoidance behavior in worms.