Channelpedia

PubMed 26050816


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Cav1.2 , Kir6.2 , Kv1.4 , Kv1.5 , Kv3.1 , Kv4.2 , Nav1 , Nav1.1 , Nav1.5



Title: Comparison of formaldehyde and methanol fixatives used in the detection of ion channel proteins in isolated rat ventricular myocytes by immunofluorescence labelling and confocal microscopy.

Authors: T T Yamanushi, M R Boyett, Y Yamamoto, H Ohsaki, E Hirakawa, H Dobrzynski

Journal, date & volume: Folia Morphol. (Warsz), 2015 , 74, 258-61

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/26050816


Abstract
In this study, a fixation protocol using a 10% neutral buffered formalin (FA) solution and another protocol using a methanol (MeOH) solution were compared for detection of ion channels, Kv1.5, Kv4.2, Cav1.2, Kir6.2, Nav1.5 and Nav1.1 in rat myocytes by immunolabelling. Kv1.5 and Kv4.2 at intercalated discs and Cav1.2 at transverse tubules were not detected by FA but were detected by MeOH. Kir6.2 at transverse tubules and Nav1.5 at sarcolemma were detected by FA but not by MeOH. It is suggested that both FA and MeOH fixation protocols should be used for the detection of cardiac ion channels by immunolabelling.