Referenced in: none

Automatically associated channels: Cav3.1

Title: [Role of calmodulin-dependent protein kinase II in bupivacaine hydrochloride-induced injury of SH-SY5Y cells].

Authors: Xianjie Wen, Jiying Zhong, Tao Zhang, Xiaohong Lai, Hongzhen Liu, Hanbing Wang, Chengxiang Yang

Journal, date & volume: Nan Fang Yi Ke Da Xue Xue Bao, 2015 Aug , 35, 1133-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/26277509

Abstract
To investigate the effect of KN93, a calmodulin-dependent protein kinase II (CaMK II) inhibitor, on SH-SY5Y cell injury induced by bupivacaine hydrochloride.SH-SY5Y cells exposed for 24 h to 1 mmol/L KN93, 1 mmol/L bupivacaine hydrochloride, or both were examined for morphological changes and Cav3.1 protein expressions using Western blotting. The vitality and apoptosis rate of the cells at different time points during the exposures were assessed with MTT assay and flow cytometry, respectively.Bupivacaine hydrochloride exposure caused obvious cell morphologial changes, reduced cell viability, increased cell apoptosis, and enhanced Cav3.1 protein expression. All these changes were partly reversed by treatment of the cells with 1 mmol/L KN93.CaMKII may play a role in bupivacaine hydrochloride-induced SH-SY5Y cells injury, which is related with upregulated Cav3.1 protein expression.