Referenced in: none

Automatically associated channels: TRP

Title: Competing Lipid-Protein and Protein-Protein Interactions Determine Clustering and Gating Patterns in the Potassium Channel from Streptomyces lividans (KcsA).

Authors: M Luisa Molina, A Marcela Giudici, José A Poveda, Gregorio Fernández-Ballester, Estefania Montoya, M Lourdes Renart, Asia M Fernández, José A Encinar, Gloria Riquelme, Andrés Morales, José M González-Ros

Journal, date & volume: J. Biol. Chem., 2015 Oct 16 , 290, 25745-55

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/26336105

Abstract
There is increasing evidence to support the notion that membrane proteins, instead of being isolated components floating in a fluid lipid environment, can be assembled into supramolecular complexes that take part in a variety of cooperative cellular functions. The interplay between lipid-protein and protein-protein interactions is expected to be a determinant factor in the assembly and dynamics of such membrane complexes. Here we report on a role of anionic phospholipids in determining the extent of clustering of KcsA, a model potassium channel. Assembly/disassembly of channel clusters occurs, at least partly, as a consequence of competing lipid-protein and protein-protein interactions at nonannular lipid binding sites on the channel surface and brings about profound changes in the gating properties of the channel. Our results suggest that these latter effects of anionic lipids are mediated via the Trp(67)-Glu(71)-Asp(80) inactivation triad within the channel structure and its bearing on the selectivity filter.