Referenced in: none

Automatically associated channels: Nav1.7

Title: Voltage-gated sodium channel expressed in cultured human smooth muscle cells: involvement of SCN9A.

Authors: Taisuke Jo, Taiji Nagata, Haruko Iida, Hiroyuki Imuta, Kuniaki Iwasawa, Ji Ma, Kei Hara, Masao Omata, Ryozo Nagai, Hajime Takizawa, Takahide Nagase, Toshiaki Nakajima

Journal, date & volume: FEBS Lett., 2004 Jun 4 , 567, 339-43

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/15178348

Abstract
Voltage-gated Na(+) channel (I(Na)) is expressed under culture conditions in human smooth muscle cells (hSMCs) such as coronary myocytes. The aim of this study is to clarify the physiological, pharmacological and molecular characteristics of I(Na) expressed in cultured hSMCs obtained from bronchus, main pulmonary and coronary artery. I(Na), was recorded in these hSMCs and inhibited by tetrodotoxin (TTX) with an IC(50) value of approximately 10 nM. Reverse transcriptase/polymerase chain reaction (RT-PCR) analysis of mRNA showed the prominent expression of transcripts for SCN9A, which was consistent with the results of real-time quantitative RT-PCR. These results provide novel evidence that TTX-sensitive Na(+) channel expressed in cultured hSMCs is mainly composed of Na(v)1.7.