Referenced in: none

Automatically associated channels: Nav1.5

Title: [Desmoplakin expression silencing affects cardiac voltage-gated sodium channel Nav1.5 in HL-1 cells].

Authors: Qianhuan Zhang, Chunyu Deng, Fang Rao, Xiaoying Liu, Liping Mai, Jiening Zhu, Honghong Tan, Shulin Wu

Journal, date & volume: Nan Fang Yi Ke Da Xue Xue Bao, 2013 Jul , 33, 983-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23895837

Abstract
To investigate the association of desmoplakin with the distribution and function of Nav1.5 by RNA silencing technology in HL-1 cells.HL-1 cells with desmoplakin expression suppression by RNA silencing were examined for desmoplakin and Nav1.5 protein expressions by Western blotting, and the distribution and co-location of desmoplakin and Nav1.5 protein were detected by immunofluorescence staining. Patch-clamp recording was applied to analyze the changes in whole-cell sodium current after desmoplakin silencing.Compared with the untreated group and negative control group, the cells with desmoplakin silencing showed obviously reduced expressions of desmoplakin and Nav1.5 proteins. Co-localization of desmoplakin and Nav1.5 was detected at cell-cell contact in untreated and control conditions, and desmoplakin expression silencing induced a drastic redistribution of Nav1.5 with decreased peak current density (156.3∓6.2 vs 41.8∓3.1, n=6, P<0.05), a shift in voltage dependence of steady-state inactivation (-42 mV vs -61 mV, n=5, P<0.05), and prolonged time of recovery from inactivation.Desmoplakin silencing caused redistribution of Nav1.5 protein and also changes in its electrophysiological properties in HL-1 cells.