Referenced in: none

Automatically associated channels: TRP , TRPC , TRPC6

Title: The canonical transient receptor potential 6 (TRPC6) channel is sensitive to extracellular pH in mouse platelets.

Authors: Alejandro Berna-Erro, Letizia Albarrán, Natalia Dionisio, Pedro C Redondo, Nieves Alonso, Luis J Gómez, Ginés M Salido, Juan A Rosado

Journal, date & volume: Blood Cells Mol. Dis., 2014 Feb-Mar , 52, 108-15

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24074949

Abstract
The canonical transient receptor potential-6 (TRPC6) is a receptor-activated non-selective Ca(2+) channel regulated by a variety of modulators such as diacylglycerol, Ca(2+)/calmodulin or phosphorylation. The present study is aimed to investigate whether different situations, such as acidic pH, exposure to reactive oxygen species (ROS) or hypoxic-like conditions modulate TRPC6 channel function. Here we show normal aggregation and Ca(2+) mobilization stimulated by thrombin in TRPC6 KO platelets; however, OAG (1-oleoyl-2-acetyl-sn-glycerol)-evoked Ca(2+) entry was attenuated in the absence of TRPC6. Exposure of mouse platelets to acidic pH resulted in abolishment of thrombin-evoked aggregation and attenuated platelet aggregation induced by thapsigargin (TG) or OAG. Both OAG-induced Ca(2+) entry and platelet aggregation were greatly attenuated in cells expressing TRPC6 channels. Exposure of platelets to H2O2 or deferoxamine did not clearly alter thrombin, TG or OAG-induced platelet aggregation. Our results indicate that TRPC6 is sensitive to acidic pH but not to exposure to ROS or hypoxic-like conditions, which might be involved in the pathogenesis of the altered platelet responsiveness to DAG-generating agonists in disorders associated to acidic pH.