Channelpedia

PubMed 24440986


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: ClvC3 , ClvC4



Title: ClC-3 chloride channel/antiporter defect contributes to inflammatory bowel disease in humans and mice.

Authors: Lin-Yan Huang, Qing He, Si-Jia Liang, Ying-Xue Su, Li-Xiong Xiong, Qian-Qian Wu, Qin-Yan Wu, Jing Tao, Jian-Ping Wang, Yong-Bo Tang, Xiao-Fei Lv, Jie Liu, Yong-Yuan Guan, Rui-Ping Pang, Jia-Guo Zhou

Journal, date & volume: Gut, 2014 Oct , 63, 1587-95

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24440986


Abstract
ClC-3 channel/antiporter plays a critical role in a variety of cellular activities. ClC-3 has been detected in the ileum and colon.To determine the functions of ClC-3 in the gastrointestinal tract.After administration of dextran sulfate sodium (DSS) or 2,4,6-trinitrobenzenesulfonic acid (TNBS), intestines from ClC-3-/- and wild-type mice were examined by histological, cellular, molecular and biochemical approaches. ClC-3 expression was determined by western blot and immunostaining.ClC-3 expression was reduced in intestinal tissues from patients with UC or Crohn's disease and from mice treated with DSS. Genetic deletion of ClC-3 increased the susceptibility of mice to DSS- or TNBS-induced experimental colitis and prevented intestinal recovery. ClC-3 deficiency promoted DSS-induced apoptosis of intestinal epithelial cells through the mitochondria pathway. ClC-3 interacts with voltage-dependent anion channel 1, a key player in regulation of mitochondria cytochrome c release, but DSS treatment decreased this interaction. In addition, lack of ClC-3 reduced the numbers of Paneth cells and impaired the expression of antimicrobial peptides. These alterations led to dysfunction of the epithelial barrier and invasion of commensal bacteria into the mucosa.A defect in ClC-3 may contribute to the pathogenesis of IBD by promoting intestinal epithelial cell apoptosis and Paneth cell loss, suggesting that modulation of ClC-3 expression might be a new strategy for the treatment of IBD.