Channelpedia

PubMed 24904058


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv2.1



Title: Amigo adhesion protein regulates development of neural circuits in zebrafish brain.

Authors: Xiang Zhao, Juha Kuja-Panula, Maria Sundvik, Yu-Chia Chen, Vilma Aho, Marjaana A Peltola, Tarja Porkka-Heiskanen, Pertti Panula, Heikki Rauvala

Journal, date & volume: J. Biol. Chem., 2014 Jul 18 , 289, 19958-75

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24904058


Abstract
The Amigo protein family consists of three transmembrane proteins characterized by six leucine-rich repeat domains and one immunoglobulin-like domain in their extracellular moieties. Previous in vitro studies have suggested a role as homophilic adhesion molecules in brain neurons, but the in vivo functions remain unknown. Here we have cloned all three zebrafish amigos and show that amigo1 is the predominant family member expressed during nervous system development in zebrafish. Knockdown of amigo1 expression using morpholino oligonucleotides impairs the formation of fasciculated tracts in early fiber scaffolds of brain. A similar defect in fiber tract development is caused by mRNA-mediated expression of the Amigo1 ectodomain that inhibits adhesion mediated by the full-length protein. Analysis of differentiated neural circuits reveals defects in the catecholaminergic system. At the behavioral level, the disturbed formation of neural circuitry is reflected in enhanced locomotor activity and in the inability of the larvae to perform normal escape responses. We suggest that Amigo1 is essential for the development of neural circuits of zebrafish, where its mechanism involves homophilic interactions within the developing fiber tracts and regulation of the Kv2.1 potassium channel to form functional neural circuitry that controls locomotion.