Referenced in: none

Automatically associated channels: SK2

Title: Coassembly and coupling of SK2 channels and mGlu5 receptors.

Authors: Gloria García-Negredo, David Soto, Javier Llorente, Xavier Morató, Koen M O Galenkamp, Maricel Gómez-Soler, Víctor Fernández-Dueñas, Masahiko Watanabe, John P Adelman, Ryuichi Shigemoto, Yugo Fukazawa, Rafael Lujan, Francisco Ciruela

Journal, date & volume: J. Neurosci., 2014 Oct 29 , 34, 14793-802

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/25355231

Abstract
Group I metabotropic glutamate (mGlu) receptors regulate hippocampal CA1 pyramidal neuron excitability via Ca(2+) wave-dependent activation of small-conductance Ca(2+)-activated K(+) (SK) channels. Here, we show that mGlu5 receptors and SK2 channels coassemble in heterologous coexpression systems and in rat brain. Further, in cotransfected cells or rat primary hippocampal neurons, mGlu5 receptor stimulation activated apamin-sensitive SK2-mediated K(+) currents. In addition, coexpression of mGlu5 receptors and SK2 channels promoted plasma membrane targeting of both proteins and correlated with increased mGlu5 receptor function that was unexpectedly blocked by apamin. These results demonstrate a reciprocal functional interaction between mGlu5 receptors and SK2 channels that reflects their molecular coassembly.