Referenced in: none

Automatically associated channels: TRP , TRPV , TRPV1

Title: A TRPV channel in Drosophila motor neurons regulates presynaptic resting Ca2+ levels, synapse growth, and synaptic transmission.

Authors: Ching-On Wong, Kuchuan Chen, Yong Qi Lin, Yufang Chao, Lita Duraine, Zhongmin Lu, Wan Hee Yoon, Jeremy M Sullivan, Geoffrey T Broadhead, Charlotte J Sumner, Thomas E Lloyd, Gregory T Macleod, Hugo J Bellen, Kartik Venkatachalam

Journal, date & volume: Neuron, 2014 Nov 19 , 84, 764-77

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/25451193

Abstract
Presynaptic resting Ca(2+) influences synaptic vesicle (SV) release probability. Here, we report that a TRPV channel, Inactive (Iav), maintains presynaptic resting [Ca(2+)] by promoting Ca(2+) release from the endoplasmic reticulum in Drosophila motor neurons, and is required for both synapse development and neurotransmission. We find that Iav activates the Ca(2+)/calmodulin-dependent protein phosphatase calcineurin, which is essential for presynaptic microtubule stabilization at the neuromuscular junction. Thus, loss of Iav induces destabilization of presynaptic microtubules, resulting in diminished synaptic growth. Interestingly, expression of human TRPV1 in Iav-deficient motor neurons rescues these defects. We also show that the absence of Iav causes lower SV release probability and diminished synaptic transmission, whereas Iav overexpression elevates these synaptic parameters. Together, our findings indicate that Iav acts as a key regulator of synaptic development and function by influencing presynaptic resting [Ca(2+)].