Referenced in: none

Automatically associated channels: TRP

Title: The anoctamin family channel subdued mediates thermal nociception in Drosophila.

Authors: Wijeong Jang, Ji Young Kim, Shanyu Cui, Juyeon Jo, Byoung-Cheol Lee, Yeonwoo Lee, Ki-Sun Kwon, Chul-Seung Park, Changsoo Kim

Journal, date & volume: J. Biol. Chem., 2015 Jan 23 , 290, 2521-8

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/25505177

Abstract
Calcium-permeable and thermosensitive transient receptor potential (TRP) channels mediate the nociceptive transduction of noxious temperature in Drosophila nociceptors. However, the underlying molecular mechanisms are not completely understood. Here we find that Subdued, a calcium-activated chloride channel of the Drosophila anoctamin family, functions in conjunction with the thermo-TRPs in thermal nociception. Genetic analysis with deletion and the RNAi-mediated reduction of subdued show that subdued is required for thermal nociception in nociceptors. Further genetic analysis of subdued mutant and thermo-TRP mutants show that they interact functionally in thermal nociception. We find that Subdued expressed in heterologous cells mediates a strong chloride conductance in the presence of both heat and calcium ions. Therefore, our analysis suggests that Subdued channels may amplify the nociceptive neuronal firing that is initiated by thermo-TRP channels in response to thermal stimuli.