PubMed 25587116

Referenced in Channelpedia wiki pages of: none

Automatically associated channels: ClvC4 , ClvC5

Title: Prolactin stimulates sodium and chloride ion channels in A6 renal epithelial cells.

Authors: Megan M Greenlee, Jeremiah D Mitzelfelt, Billie Jeanne Duke, Otor K Al-Khalili, Hui-Fang Bao, Douglas C Eaton

Journal, date & volume: Am. J. Physiol. Renal Physiol., 2015 Jan 13 , , ajprenal.00270.2014

PubMed link:

Many hormonal pathways contribute to the regulation of renal epithelial sodium channel (ENaC) function, a key process for maintaining blood volume and controlling blood pressure. In the present study, we examined whether the peptide hormone prolactin (PRL) regulates ENaC function in renal epithelial cells (A6). Basolateral application of several different concentrations of PRL dramatically stimulated the transepithelial current in A6 cells, increasing both amiloride-sensitive (ENaC) and amiloride-insensitive currents. Using cell-attached patch clamp, we determined that PRL increased both the number (N) and open probability (Po) of ENaC present in the apical membrane. Inhibition of PKA with H-89 abolished the effect of PRL on amiloride-sensitive and insensitive transepithelial currents and eliminated the increase in ENaC NPo with PRL exposure. PRL also increased cAMP in A6 cells, consistent with signaling through the cAMP-dependent PKA pathway. We also identified that PRL induced activity of a 2-pS anion channel with outward rectification, electrophysiological properties consistent with ClC4 or ClC5. RT-PCR only detected ClC4, but not ClC5 transcripts. Here, we show for the first time that PRL activates sodium and chloride transport in renal epithelial cells via ENaC and ClC4.