Channelpedia

PubMed 23973818


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kir6.1



Title: Evidences for an ATP-sensitive potassium channel (KATP) in muscle and fat body mitochondria of insect.

Authors: Malgorzata Slocinska, Jan Lubawy, Wieslawa Jarmuszkiewicz, Grzegorz Rosinski

Journal, date & volume: J. Insect Physiol., 2013 Nov , 59, 1125-32

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23973818


Abstract
In the present study, we describe the existence of mitochondrial ATP-dependent K(+) channel (mitoKATP) in two different insect tissues, fat body and muscle of cockroach Gromphadorhina coquereliana. We found that pharmacological substances known to modulate potassium channel activity influenced mitochondrial resting respiration. In isolated mitochondria oxygen consumption increased by about 13% in the presence of potassium channel openers (KCOs) such as diazoxide and pinacidil. The opening of mitoKATP was reversed by glibenclamide (potassium channel blocker) and 1 mM ATP. Immunological studies with antibodies raised against the Kir6.1 and SUR1 subunits of the mammalian ATP-sensitive potassium channel, indicated the existence of mitoKATP in insect mitochondria. MitoKATP activation by KCOs resulted in a decrease in superoxide anion production, suggesting that protection against mitochondrial oxidative stress may be a physiological role of mitochondrial ATP-sensitive potassium channel in insects.