Referenced in: none

Automatically associated channels: TRP , TRPA , TRPA1

Title: Insect pheromone behavior: fruit fly.

Authors: Daisuke Yamamoto, Soh Kohatsu, Masayuki Koganezawa

Journal, date & volume: Methods Mol. Biol., 2013 , 1068, 261-72

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24014368

Abstract
The amenability to genetics of Drosophila melanogaster has made this organism one of the best-suited models for studying the neurobiology of pheromone-guided behavior. Single-male assays use the minigene encoding the thermosensitive channel dTrpA1 to activate neurons expressing fruitless (fru), a major courtship regulator gene, and thereby induce most of the elementary courtship acts in a solitary male exposed to temperature increase. Tethered male assays allow Ca(2+)-imaging of neuronal activities of a male fly displaying courtship behavior on a treadmill when stimulated with a female or pheromones. Here we describe technical details of these assays.