Referenced in: none

Automatically associated channels: TRP , TRPC , TRPC5

Title: Tumor endothelial expression of P-glycoprotein upon microvesicular transfer of TrpC5 derived from adriamycin-resistant breast cancer cells.

Authors: YePing Dong, Qiongxi Pan, Li Jiang, Zhen Chen, Fangfang Zhang, YanJun Liu, Hui Xing, Mei Shi, Jiao Li, XiYuan Li, YaoDan Zhu, Yun Chen, Iain C Bruce, Jian Jin, Xin Ma

Journal, date & volume: Biochem. Biophys. Res. Commun., 2014 Mar 28 , 446, 85-90

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24582564

Abstract
Treatment of carcinoma commonly fails due to chemoresistance. Studies have shown that endothelial cells acquire resistance via the tumor microenvironment. Microvesicle (MV) shedding from the cell membrane to the microenvironment plays an important role in communication between cells. The aim of the present study was to determine whether MCF-7 adriamycin-resistant cells (MCF-7/ADM) shed MVs that alter the characteristics of human microvessel endothelial cells (HMECs). MVs from tumor cells transferred a Ca(2+)-permeable channel TrpC5 to HMECs, inducing the expression of P-glycoprotein (P-gp) by activation of the transcription factor NFATc3 (nuclear factor of activated T cells isoform c3). Expression of the mdr1 gene was blocked by the TrpC5-blocking antibody T5E3, and the production of P-gp in HMECs was reduced by blockade of TrpC5. Thus, we postulate that endothelial cells acquire the resistant protein upon exposure to TrpC5-containg MVs in the microenvironment, and express P-gp in the TrpC5-NFATc3 signal pathway.