Referenced in: none

Automatically associated channels: Nav1.5

Title: Screening for voltage-gated sodium channel interacting peptides.

Authors: Er Meng, Tian-Fu Cai, Hui Zhang, Si Tang, Meng-Jie Li, Wen-Ying Li, Peng-Fei Huang, Kai Liu, Lei Wu, Ling-Yun Zhu, Long Liu, Kuan Peng, Xian-Dong Dai, Hui Jiang, Xiong-Zhi Zeng, Song-Ping Liang, Dong-Yi Zhang

Journal, date & volume: Sci Rep, 2014 , 4, 4569

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24691553

Abstract
The voltage-gated sodium channel (VGSC) interacting peptide is of special interest for both basic research and pharmaceutical purposes. In this study, we established a yeast-two-hybrid based strategy to detect the interaction(s) between neurotoxic peptide and the extracellular region of VGSC. Using a previously reported neurotoxin JZTX-III as a model molecule, we demonstrated that the interactions between JZTX-III and the extracellular regions of its target hNav1.5 are detectable and the detected interactions are directly related to its activity. We further applied this strategy to the screening of VGSC interacting peptides. Using the extracellular region of hNav1.5 as the bait, we identified a novel sodium channel inhibitor SSCM-1 from a random peptide library. This peptide selectively inhibits hNav1.5 currents in the whole-cell patch clamp assays. This strategy might be used for the large scale screening for target-specific interacting peptides of VGSCs or other ion channels.