Referenced in: none

Automatically associated channels: ClC1 , ClC4

Title: Nongenomic actions of progesterone and 17β-estradiol on the chloride conductance of skeletal muscle.

Authors: James A Burge, Michael G Hanna, Stephanie Schorge

Journal, date & volume: Muscle Nerve, 2013 Oct , 48, 589-91

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23625574

Abstract
Myotonia congenita, caused by mutations in ClC-1, tends to be more severe in men and is often exacerbated by pregnancy.We performed whole-cell patch clamp of mouse muscle chloride currents in the absence/presence of 100 μM progesterone or 17β-estradiol.100 μM progesterone rapidly and reversibly shifted the ClC-1 activation curve of mouse skeletal muscle (V50 changed from -52.6 ± 9.3 to +35.5 ± 6.7; P < 0.01) and markedly reduced chloride currents at depolarized potentials. 17β-estradiol at the same concentration had a similar but smaller effect (V50 change from -57.2 ± 7.6 to -40.5 ± 9.8; P < 0.05). 1 μM progesterone produced no significant effect.Although the data support the existence of a nongenomic mechanism in mammalian skeletal muscle through which sex hormones at high concentration can rapidly modulate ClC-1, the influence of hormones on muscle excitability in vivo remains an open question.