Referenced in: none

Automatically associated channels: K2P , TREK1

Title: Endothelial TWIK-related potassium channel-1 (TREK1) regulates immune-cell trafficking into the CNS.

Authors: Stefan Bittner, Tobias Ruck, Michael K Schuhmann, Alexander M Herrmann, Hamid Moha Ou Maati, Nicole Bobak, Kerstin Göbel, Friederike Langhauser, David Stegner, Petra Ehling, Marc Borsotto, Hans-Christian Pape, Bernhard Nieswandt, Christoph Kleinschnitz, Catherine Heurteaux, Hans-Joachim Galla, Thomas Budde, Heinz Wiendl, Sven G Meuth

Journal, date & volume: Nat. Med., 2013 Sep , 19, 1161-5

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23933981

Abstract
The blood-brain barrier (BBB) is an integral part of the neurovascular unit (NVU). The NVU is comprised of endothelial cells that are interconnected by tight junctions resting on a parenchymal basement membrane ensheathed by pericytes, smooth muscle cells and a layer of astrocyte end feet. Circulating blood cells, such as leukocytes, complete the NVU. BBB disruption is common in several neurological diseases, but the molecular mechanisms involved remain largely unknown. We analyzed the role of TWIK-related potassium channel-1 (TREK1, encoded by KCNK2) in human and mouse endothelial cells and the BBB. TREK1 was downregulated in endothelial cells by treatment with interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α). Blocking TREK1 increased leukocyte transmigration, whereas TREK1 activation had the opposite effect. We identified altered mitogen-activated protein (MAP) kinase signaling, actin remodeling and upregulation of cellular adhesion molecules as potential mechanisms of increased migration in TREK1-deficient (Kcnk2(-/-)) cells. In Kcnk2(-/-) mice, brain endothelial cells showed an upregulation of the cellular adhesion molecules ICAM1, VCAM1 and PECAM1 and facilitated leukocyte trafficking into the CNS. Following the induction of experimental autoimmune encephalomyelitis (EAE) by immunization with a myelin oligodendrocyte protein (MOG)35-55 peptide, Kcnk2(-/-) mice showed higher EAE severity scores that were accompanied by increased cellular infiltrates in the central nervous system (CNS). The severity of EAE was attenuated in mice given the amyotrophic lateral sclerosis drug riluzole or fed a diet enriched with linseed oil (which contains the TREK-1 activating omega-3 fatty acid α-linolenic acid). These beneficial effects were reduced in Kcnk2(-/-) mice, suggesting TREK-1 activating compounds may be used therapeutically to treat diseases related to BBB dysfunction.