Referenced in: none

Automatically associated channels: Kv7.1 , Slo1

Title: Intracellular ATP binding is required to activate the slowly activating K+ channel I(Ks).

Authors: Yang Li, Junyuan Gao, Zhongju Lu, Kelli McFarland, Jingyi Shi, Kevin Bock, Ira S Cohen, Jianmin Cui

Journal, date & volume: Proc. Natl. Acad. Sci. U.S.A., 2013 Nov 19 , 110, 18922-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24190995

Abstract
Gating of ion channels by ligands is fundamental to cellular function, and ATP serves as both an energy source and a signaling molecule that modulates ion channel and transporter functions. The slowly activating K(+) channel I(Ks) in cardiac myocytes is formed by KCNQ1 and KCNE1 subunits that conduct K(+) to repolarize the action potential. Here we show that intracellular ATP activates heterologously coexpressed KCNQ1 and KCNE1 as well as I(Ks) in cardiac myocytes by directly binding to the C terminus of KCNQ1 to allow the pore to open. The channel is most sensitive to ATP near its physiological concentration, and lowering ATP concentration in cardiac myocytes results in I(Ks) reduction and action potential prolongation. Multiple mutations that suppress I(Ks) by decreasing the ATP sensitivity of the channel are associated with the long QT (interval between the Q and T waves in electrocardiogram) syndrome that predisposes afflicted individuals to cardiac arrhythmia and sudden death. A cluster of basic and aromatic residues that may form a unique ATP binding site are identified; ATP activation of the wild-type channel and the effects of the mutations on ATP sensitivity are consistent with an allosteric mechanism. These results demonstrate the activation of an ion channel by intracellular ATP binding, and ATP-dependent gating allows I(Ks) to couple myocyte energy state to its electrophysiology in physiologic and pathologic conditions.