Referenced in: none

Automatically associated channels: TRP , TRPM , TRPM4

Title: Increased β-Adrenergic Inotropy in Ventricular Myocardium From Trpm4-/- Mice.

Authors: Ilka Mathar, Miklós Kecskés, Gerry Van der Mieren, Griet Jacobs, Juan E Camacho Londoño, Sebastian Uhl, Veit Flockerzi, Thomas Voets, Marc Freichel, Bernd Nilius, Paul Herijgers, Rudi Vennekens

Journal, date & volume: Circ. Res., 2014 Jan 17 , 114, 283-94

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24226423

Abstract
The Trpm4 gene has recently been associated with several disorders, including cardiac conduction diseases and Brugada syndrome. Transient receptor potential member 4 (TRPM4) proteins constitute Ca2+ -activated, but Ca2+ -impermeable, nonselective cation channels and are expressed both in atrial and in ventricular cardiomyocytes. The physiological function of TRPM4 in the heart remains, however, incompletely understood.To establish the role of TRPM4 in cardiac muscle function.We used TRPM4 knockout mice and performed patch-clamp experiments, membrane potential measurements, microfluorometry, contractility measurements, and in vivo pressure-volume loop analysis. We demonstrate that TRPM4 proteins are functionally present in mouse ventricular myocytes and are activated on Ca2+ -induced Ca2+ release. In Trpm4(-/-) mice, cardiac muscle displays an increased β-adrenergic inotropic response both in vitro and in vivo. Measurements of action potential duration show a significantly decreased time for 50% and 90% repolarization in Trpm4(-/-) ventricular myocytes. We provide evidence that this change in action potential shape leads to an increased driving force for the L-type Ca2+ current during the action potential, which explains the altered contractility of the heart muscle.Our results show that functional TRPM4 proteins are novel determinants of the inotropic effect of β-adrenergic stimulation on the ventricular heart muscle.