Referenced in: none

Automatically associated channels: TRP , TRPV , TRPV4

Title: Ca2+ Imaging as a tool to assess TRP channel function in murine distal nephrons.

Authors: Mykola Mamenko, Oleg Zaika, Roger G O'neil, Oleh Pochynyuk

Journal, date & volume: Methods Mol. Biol., 2013 , 998, 371-84

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23529445

Abstract
Transient receptor potential (TRP) channels are expressed in almost every segment of renal nephron from the glomerulus to the inner medullary collecting duct. Serving as a route for Ca(2+) entry from the intratubular space into cells in response to external cues, TRP channels modulate water-electrolyte transport, thus determining functional properties of the renal tubule. In this chapter, we discuss technical aspects of using Ca(2+) imaging to monitor activity of TRP channels in situ, namely, in the freshly isolated distal nephrons, with a special emphasis on the mechanosensitive TRPV4 channel and its role in tubular flow sensing.