Referenced in: none

Automatically associated channels: SK3

Title: Pivotal role of the lipid Raft SK3-Orai1 complex in human cancer cell migration and bone metastases.

Authors: Aurelie Chantome, Marie Potier-Cartereau, Lucie Clarysse, Gaëlle Fromont, Séverine Marionneau-Lambot, Maxime Guéguinou, Jean-Christophe Pagès, Christine Collin, Thibauld Oullier, Alban Girault, Flavie Arbion, Jean-Pierre Haelters, Paul-Alain Jaffrès, Michelle Pinault, Pierre Besson, Virginie Joulin, Philippe Bougnoux, Christophe Vandier

Journal, date & volume: Cancer Res., 2013 Aug 1 , 73, 4852-61

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23774210

Abstract
The SK3 channel, a potassium channel, was recently shown to control cancer cell migration, a critical step in metastasis outgrowth. Here, we report that expression of the SK3 channel was markedly associated with bone metastasis. The SK3 channel was shown to control constitutive Ca(2+) entry and cancer cell migration through an interaction with the Ca(2+) channel Orai1. We found that the SK3 channel triggers an association with the Orai1 channel within lipid rafts. This localization of an SK3-Orai1 complex seemed essential to control cancer cell migration. This suggests that the formation of this complex in lipid rafts is a gain-of-function, because we showed that none of the individual proteins were able to promote the complete phenotype. We identified the alkyl-lipid Ohmline as a disrupting agent for SK3-Orai1 lipid raft localization. Upon Ohmline treatment, the SK3-Orai1 complex moved away from lipid rafts, and SK3-dependent Ca(2+) entry, migration, and bone metastases were subsequently impaired. The colocalization of SK3 and Orai1 in primary human tumors and bone metastases further emphasized the clinical relevance of our observations. Targeting SK3-Orai1 in lipid rafts may inaugurate innovative approaches to inhibit bone metastases.