Referenced in: none

Automatically associated channels: Slo1 , TRP , TRPC , TRPC1

Title: TRPC1 protein forms only one type of native store-operated channels in HEK293 cells.

Authors: Anton Skopin, Alexey Shalygin, Vladimir Vigont, Olga Zimina, Lyubov Glushankova, Galina N Mozhayeva, Elena Kaznacheyeva

Journal, date & volume: Biochimie, 2013 Feb , 95, 347-53

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23079337

Abstract
TRPC1 is a major component of store-operated calcium entry in many cell types. In our previous studies, three types of endogenous store-operated calcium channels have been described in HEK293 cells, but it remained unknown which of these channels are composed of TRPC1 proteins. Here, this issue has been addressed by performing single-channel analysis in HEK293 cells transfected with anti-TRPC1 siRNA (siTPRC1) or a TPRC1-encoding plasmid. The results show that thapsigargin-or agonist-induced calcium influx is significantly attenuated in siTRPC1-transfected HEK293 cells. TRPC1 knockdown by siRNA results in the disappearance of store-operated I(max) channels, while the properties of I(min) and I(NS) channels are unaffected. In HEK293 cells with overexpressed TRPC1 protein, the unitary current-voltage relationship of exogenous TRPC1 channels is almost linear, with a slope conductance of about 17 pS. The extrapolated reversal potential of expressed TRPC1 channels is +30 mV. Therefore, the main electrophysiological and regulatory properties of expressed TRPC1 and native I(max) channels are identical. Moreover, TRPC1 overexpression in HEK293 cells results in an increased number of store-operated I(max) channels. All these data allow us to conclude that TRPC1 protein forms native store-operated I(max) channels but is not an essential subunit for other store-operated channel types in HEK293 cells.