Referenced in: none

Automatically associated channels: TRP , TRPM , TRPM2

Title: Expression and regulation of transient receptor potential cation channel, subfamily M, member 2 (TRPM2) in human endometrium.

Authors: Hisahiko Hiroi, Mikio Momoeda, Toru Watanabe, Masanori Ito, Kazuhiro Ikeda, Ryo Tsutsumi, Yumi Hosokawa, Minako Koizumi, Fumiko Zenri, Masami Muramatsu, Yuji Taketani, Satoshi Inoue

Journal, date & volume: Mol. Cell. Endocrinol., 2013 Jan 30 , 365, 146-52

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23142700

Abstract
To identify estrogen-responsive genes, we previously isolated estrogen receptor (ER)-binding DNA fragments from human genomic DNA using a recombinant ER protein. Six DNA fragments, each including a perfect palindromic estrogen response element (ERE), were obtained. The nucleotide sequence of one of the six fragments (E1 fragment) showed that the ERE of the E1 fragment is located in the 3'-untranslated region (UTR) of transient receptor potential cation channel, subfamily M, member 2 (TRPM2). Here, we confirmed the estrogen-dependent enhancer activity of the ERE of the E1 fragment by chloramphenicol acetyltransferase assay. TRPM2 mRNA expression was investigated in human endometrium, cultured human endometrial stromal cells (ESCs), and cultured human endometrial epithelial cells (EECs) using RT-PCR. Quantitative RT-PCR revealed that TRPM2 mRNA expression in ESCs increased after 17β-estradiol (E2) treatment. This study demonstrated for the first time that TRPM2 is an estrogen-responsive gene expressed in human endometrial cells.