Channelpedia

PubMed 15272009


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: ClvC2 , ClvC4



Title: Plasmodium induces swelling-activated ClC-2 anion channels in the host erythrocyte.

Authors: Stephan M Huber, Christophe Duranton, Guido Henke, Claudia Van De Sand, Volker Heussler, Ekaterina Shumilina, Ciprian D Sandu, Valerie Tanneur, Verena Brand, Ravi S Kasinathan, Karl S Lang, Peter G Kremsner, Christian A Hübner, Marco B Rust, Karin Dedek, Thomas J Jentsch, Florian Lang

Journal, date & volume: J. Biol. Chem., 2004 Oct 1 , 279, 41444-52

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/15272009


Abstract
Intraerythrocytic growth of the human malaria parasite Plasmodium falciparum depends on delivery of nutrients. Moreover, infection challenges cell volume constancy of the host erythrocyte requiring enhanced activity of cell volume regulatory mechanisms. Patch clamp recording demonstrated inwardly and outwardly rectifying anion channels in infected but not in control erythrocytes. The molecular identity of those channels remained elusive. We show here for one channel type that voltage dependence, cell volume sensitivity, and activation by oxidation are identical to ClC-2. Moreover, Western blots and FACS analysis showed protein and functional ClC-2 expression in human erythrocytes and erythrocytes from wild type (Clcn2(+/+)) but not from Clcn2(-/-) mice. Finally, patch clamp recording revealed activation of volume-sensitive inwardly rectifying channels in Plasmodium berghei-infected Clcn2(+/+) but not Clcn2(-/-) erythrocytes. Erythrocytes from infected mice of both genotypes differed in cell volume and inhibition of ClC-2 by ZnCl(2) (1 mm) induced an increase of cell volume only in parasitized Clcn2(+/+) erythrocytes. Lack of ClC-2 did not inhibit P. berghei development in vivo nor substantially affect the mortality of infected mice. In conclusion, activation of host ClC-2 channels participates in the altered permeability of Plasmodium-infected erythrocytes but is not required for intraerythrocytic parasite survival.