Channelpedia

PubMed 23494376


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv10.1



Title: Developing a photoreactive antagonist.

Authors: Pamela M England

Journal, date & volume: Methods Mol. Biol., 2013 , 995, 121-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23494376


Abstract
Light is an exquisite reagent for controlling the activity of biological systems, often offering improved temporal and spatial resolution over strictly genetic, biochemical, or pharmacological manipulations. This chapter describes a general approach for developing small molecules that, upon irradiation with light, may be used to rapidly inactivate targeted proteins expressed on the surfaces of cells. Highlighted is ANQX, a photoreactive AMPA receptor antagonist developed to irreversibly inactivate a subtype of glutamate-gated ion channels natively expressed on neurons.