PubMed 23494376

Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv10.1

Title: Developing a photoreactive antagonist.

Authors: Pamela M England

Journal, date & volume: Methods Mol. Biol., 2013 , 995, 121-9

PubMed link:

Light is an exquisite reagent for controlling the activity of biological systems, often offering improved temporal and spatial resolution over strictly genetic, biochemical, or pharmacological manipulations. This chapter describes a general approach for developing small molecules that, upon irradiation with light, may be used to rapidly inactivate targeted proteins expressed on the surfaces of cells. Highlighted is ANQX, a photoreactive AMPA receptor antagonist developed to irreversibly inactivate a subtype of glutamate-gated ion channels natively expressed on neurons.