Referenced in: none

Automatically associated channels: Kv11.1

Title: Klotho sensitivity of the hERG channel.

Authors: Carlos Munoz, Tatsiana Pakladok, Ahmad Almilaji, Bernat Elvira, Guiscard Seebohm, Jakob Voelkl, Michael Föller, Ekaterina Shumilina, Florian Lang

Journal, date & volume: FEBS Lett., 2013 Jun 5 , 587, 1663-8

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23603386

Abstract
Klotho, a hormone and enzyme, is a powerful regulator of ageing and life span. Klotho deficiency leads to cardiac arrythmia and sudden cardiac death. We thus explored whether klotho modifies cardiac K(+)-channel hERG. Current was determined utilizing dual electrode voltage clamp and hERG protein abundance utilizing immunohistochemistry and chemiluminescence in Xenopus oocytes expressing hERG with or without klotho. Coexpression of klotho increased cell membrane hERG-protein abundance and hERG current at any given voltage without significantly modifying the voltage required to activate the channel. The effect of klotho coexpression was mimicked by recombinant klotho protein and reversed by β-glucuronidase-inhibitor D-saccharic acid-1,4-lactone.