Channelpedia

PubMed 23839156


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: KCNQ1 , Kv1.4 , Kv1.5 , Kv3.1 , Kv4.3 , Kv7.1 , Slo1



Title: A Common Structural Component for β-Subunit Mediated Modulation of Slow Inactivation in Different KV Channels.

Authors: Nathalie Strutz-Seebohm, Ulrike Henrion, Nicole Schmitt, Eric Schulze-Bahr, Guiscard Seebohm

Journal, date & volume: Cell. Physiol. Biochem., 2013 , 31, 968-80

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23839156


Abstract
Potassium channels are tetrameric proteins providing potassium selective passage through lipid embedded proteinaceous pores with highest fidelity. The selectivity results from binding to discrete potassium binding sites and stabilization of a hydrated potassium ion in a central internal cavity. The four potassium binding sites, generated by the conserved TTxGYGD signature sequence are formed by the backbone carbonyls of the amino acids TXGYG. Residues KV1.5-Val481, KV4.3-Leu368 and KV7.1- Ile 313 represent the amino acids in the X position of the respective channels.Here, we study the impact of these residues on ion selectivity, permeation and inactivation kinetics as well as the modulation by β-subunits using site-specific mutagenesis, electrophysiological analyses and molecular dynamics simulations.We identify this position as key in modulation of slow inactivation by structurally dissimilar β-subunits in different KV channels.We propose a model in which structural changes accompanying activation and β-subunit modulation allosterically constrain the backbone carbonyl oxygen atoms via the side chain of the respective X-residue in the signature sequence to reduce conductance during slow inactivation.