Referenced in: none

Automatically associated channels: ClIC1

Title: Chloride intracellular channel 1 is an important factor in the lymphatic metastasis of hepatocarcinoma.

Authors: Rong-kuan Li, Jun Zhang, Yu-hong Zhang, Miao-Ling Li, Mei Wang, Jian-wu Tang

Journal, date & volume: Biomed. Pharmacother., 2012 Apr , 66, 167-72

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22440897

Abstract
We have previously demonstrated that chloride intracellular channel 1 (CLIC1) is involved in the lymphatic metastasis of tumors. In this study, a self-designed shRNA sequence of mouse CLIC1 gene was synthesized and inserted into a pGPU6/GFP/Neo plasmid, then stably transfected into mouse hepatic carcinoma cell line Hca-F cells to down-regulate the expression of CLIC1 gene. The levels of expression of CLIC1 mRNA and protein were detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot (WB) analysis, respectively. The down-regulation of CLIC1 enhanced proliferative activity, increased the ratio of G2/M and decreased percentage of apoptosis. In addition, the capability of migration and invasion decreased significantly. The results indicate that CLIC1 is a critical factor in the development of lymphatic metastasis.