Referenced in: none

Automatically associated channels: TRP , TRPC , TRPC1 , TRPC3

Title: Interleukin-13 enhanced Ca2+ oscillations in airway smooth muscle cells.

Authors: Hisako Matsumoto, Yutaka Hirata, Kojiro Otsuka, Toshiyuki Iwata, Aya Inazumi, Akio Niimi, Isao Ito, Emiko Ogawa, Shigeo Muro, Hiroaki Sakai, Kazuo Chin, Yoshitaka Oku, Michiaki Mishima

Journal, date & volume: Cytokine, 2012 Jan , 57, 19-24

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22078634

Abstract
Physiological mechanisms associated with interleukin-13 (IL-13), a key cytokine in asthma, in intracellular Ca(2+) signaling in airway smooth muscle cells (ASMCs) remain unclear. The aim of this study was to assess effects of IL-13 on Ca(2+) oscillations in response to leukotriene D4 (LTD4) in human cultured ASMCs. LTD4-induced Ca(2+) oscillations in ASMCs pretreated with IL-13 were imaged by confocal microscopy. mRNA expressions of cysteinyl leukotriene 1 receptors (CysLT1R), CD38, involved with the ryanodine receptors (RyR) system, and transient receptor potential canonical (TRPC), involved with store-operated Ca(2+) entry (SOCE), were determined by real-time PCR. In IL-13-pretreated ASMCs, frequency of LTD4-induced Ca(2+) oscillations and number of oscillating cells were significantly increased compared with untreated ASMCs. Both xestospongin C, a specific inhibitor of inositol 1,4,5-triphosphate receptors (IP(3)R), and ryanodine or ruthenium red, inhibitors of RyR, partially blocked LTD4-induced Ca(2+) oscillations. Ca(2+) oscillations were almost completely inhibited by 50 μM of 2-aminoethoxydiphenyl borate (2-APB), which dominantly blocks SOCE but not IP(3)R at this concentration. Pretreatment with IL-13 increased the mRNA expressions of CysLT1R and CD38, but not of TRPC1 and TRPC3. We conclude that IL-13 enhances frequency of LTD4-induced Ca(2+) oscillations in human ASMCs, which may be cooperatively modulated by IP(3)R, RyR systems and possibly by SOCE.