Channelpedia

PubMed 22101010


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: TRP , TRPV , TRPV4



Title: Phosphorylation on the Ser 824 residue of TRPV4 prefers to bind with F-actin than with microtubules to expand the cell surface area.

Authors: Sung Hwa Shin, Eun Jeoung Lee, Sunghee Hyun, Jaesun Chun, Yangmi Kim, Sang Sun Kang

Journal, date & volume: Cell. Signal., 2012 Mar , 24, 641-51

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22101010


Abstract
Previously, we demonstrated that the transient receptor potential vanilloid 4 (TRPV4) cation channel, a member of the TRP vanilloid subfamily, is one of the serum glucocorticoid-induced protein kinase1 (SGK1) authentic substrate proteins, and that the Ser 824 residue of TRPV4 is phosphorylated by SGK1. In this study, we demonstrated that phosphorylation on the Ser 824 residue of TRPV4 is required for its interaction with F-actin, using TRPV4 mutants (S824D; a phospho-mimicking TRPV4 mutant and S824A; a non-phosphorylatable TRPV4 mutant) and its proper subcellular localization. Additionally, we noted that the phosphorylation of the Ser824 residue promotes its single channel activity, Ca(2+) influx, protein stability, and cell surface area (expansion of plasma membrane).