Channelpedia

PubMed 22261053


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv1.3 , SK1



Title: Modeling the binding of three toxins to the voltage-gated potassium channel (Kv1.3).

Authors: Rong Chen, Anna Robinson, Dan Gordon, Shin-Ho Chung

Journal, date & volume: Biophys. J., 2011 Dec 7 , 101, 2652-60

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22261053


Abstract
The conduction properties of the voltage-gated potassium channel Kv1.3 and its modes of interaction with several polypeptide venoms are examined using Brownian dynamics simulations and molecular dynamics calculations. Employing an open-state homology model of Kv1.3, we first determine current-voltage and current-concentration curves and ascertain that simulated results accord with experimental measurements. We then investigate, using a molecular docking method and molecular dynamics simulations, the complexes formed between the Kv1.3 channel and several Kv-specific polypeptide toxins that are known to interfere with the conducting mechanisms of several classes of voltage-gated K(+) channels. The depths of potential of mean force encountered by charybdotoxin, α-KTx3.7 (also known as OSK1) and ShK are, respectively, -19, -27, and -25 kT. The dissociation constants calculated from the profiles of potential of mean force correspond closely to the experimentally determined values. We pinpoint the residues in the toxins and the channel that are critical for the formation of the stable venom-channel complexes.