Referenced in: none

Automatically associated channels: TRP , TRPC , TRPC6

Title: Mouse transient receptor potential channel 6: role in hemostasis and thrombogenesis.

Authors: Enma V Paez Espinosa, John P Murad, Harold J Ting, Fadi T Khasawneh

Journal, date & volume: Biochem. Biophys. Res. Commun., 2012 Jan 13 , 417, 853-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22206677

Abstract
Although changes in the intracellular levels of calcium (Ca(2+)) are a central step in platelet activation, the underlying mechanism of Ca(2+) entry is still unclear. Previous studies have demonstrated that TRPC6, a member of the canonical transient receptor potential channel (TRPC) family is expressed in platelets in a significant amount, and is predominantly found on the plasma membrane. Based on these considerations, we hypothesized that TRPC6 plays a critical role in platelet function. To characterize the role of TRPC6 in platelet function in vivo, we employed a genetic approach, subjecting TRPC6 knockout mice to the tail bleeding time test and a carotid artery injury thrombosis model. We found that TRPC6-deficient animals displayed a prolonged bleeding time, and an increased time for occlusion of the injured carotid artery, compared to their wild-type littermates. Taken together, our data demonstrate for the first time, that TRPC6 deletion in mice results in defects in hemostasis and protection against thrombogenesis, suggesting a vital role in platelet function. Furthermore, TRPC6 may define a new therapeutic target for managing multiple thrombosis-based disorders.