Referenced in: none

Automatically associated channels: Cav1.2

Title: Protein kinase D regulates the human cardiac L-type voltage-gated calcium channel through serine 1884.

Authors: Yusuke Aita, Nagomi Kurebayashi, Shigehisa Hirose, Andrés D Maturana

Journal, date & volume: FEBS Lett., 2011 Dec 15 , 585, 3903-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22100296

Abstract
Protein kinase D (PKD) regulates the activity of the L-type calcium channel in rat ventricular cardiomyocytes. However, the functional target residues of PKD on the L-type calcium channel remain to be identified. Our aim was to identify the functional phosphorylation sites of PKD on the human L-type calcium channel. The pore subunit of the human CaV1.2 (hCaV1.2) was stably expressed in HEK293 cells. Both the expression of a dominant-negative mutant of PKD and the mutation of serine 1884 but not serine 1930, putative targets of PKD, strongly reduced L-type calcium currents and single channel activity without affecting the channel's expression at the plasma membrane. Our results suggest that serine 1884 is essential for the regulation of hCaV1.2 by PKD.