Referenced in: none

Automatically associated channels: Kir2.2

Title: Role of Kir2.2 in hypercapnic ventilatory response during postnatal development of mouse.

Authors: Yoshitaka Oyamada, Kazuhiro Yamaguchi, Michie Murai, Haruhiko Hakuno, Akitoshi Ishizaka

Journal, date & volume: , 2005 Feb 15 , 145, 143-51

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/15705530

Abstract
In order to determine the role of Kir2.2 in the hypercapnic ventilatory response (HCVR) during postnatal development, we measured the response of the Kir2.2-knockout (Kir2.2-/-) mouse in an unanesthetized unrestrained state by means of pressure plethysmography on postnatal days 9-10, 14-15 and 18, and compared the response with that in its wild counterpart, the FVB mouse. We also examined developmental changes in m-RNA expression of Kir2.2 in the brainstem of the FVB mouse using quantitative real-time PCR assay. Kir2.2-/- exhibited a smaller increase in tidal volume and minute ventilation volume than the FVB mouse in response to hypercapnic challenge on days 14-15. Meanwhile, the FVB mouse showed a transient increase in m-RNA expression of Kir2.2 in the brainstem on days 14-15. These findings suggest that Kir2.2 in the brainstem plays a transient role in HCVR, possibly through central ventilatory chemosensitivity, during postnatal development.